Perspectives on Unidirectional versus Divergent Transcription

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Perspectives on Unidirectional versus Divergent Transcription.

Sascha H.C. Duttke,1,8 Scott A. Lacadie,4,8 Mahmoud M. Ibrahim,4,5 Christopher K. Glass,2,3 David L. Corcoran,7 Christopher Benner,6 Sven Heinz,2,6 James T. Kadonaga,1,* and Uwe Ohler4,5,* 1Section of Molecular Biology 2Department of Cellular and Molecular Medicine 3Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA 4Berlin Institute for Medic...

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Divergent RNA transcription

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Divergent transcription from active promoters.

Transcription initiation by RNA polymerase II (RNAPII) is thought to occur unidirectionally from most genes. Here, we present evidence of widespread divergent transcription at protein-encoding gene promoters. Transcription start site-associated RNAs (TSSa-RNAs) nonrandomly flank active promoters, with peaks of antisense and sense short RNAs at 250 nucleotides upstream and 50 nucleotides downstr...

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Conservation of divergent transcription in fungi.

The comparison of fully sequenced genomes enables the study of selective constraints that determine genome organisation. We show that, in fungi, adjacent divergently transcribed (<---->) genes are more conserved in orientation than convergent (--><--) or co-oriented (-->-->) gene pairs. Furthermore, the time divergent orientation of two genes is conserved correlates with the degree of their co-...

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ژورنال

عنوان ژورنال: Molecular Cell

سال: 2015

ISSN: 1097-2765

DOI: 10.1016/j.molcel.2015.10.014